Journal: Journal of Gastrointestinal Cancer

Article Title: Bioinformatics Analysis Reveals the Role of DLGAP4 in the Development and Progression of Hepatocellular Carcinoma

doi: 10.1007/s12029-025-01359-w

Figure Lengend Snippet: Expression and clinicopathological characteristics of DLGAP4 in HCC ( a ) The expression of DLGAP4 in pan-cancer. ( b ) Volcano plot of DGEs in TCGA datasets, the red dots represent up-regulated genes, the blue dots represent down-regulated genes, and the grey dots represent genes with no significant difference in expression. The screening criteria are set to P < 0.0001, |log2FC|≥2. ( c ) DLGAP4 levels across tumor/normal specimens from TCGA; ( d ) DLGAP4 levels in paired tumor and normal samples (TCGA); ( e ) ROC curve analysis for DLGAP4 expression in tumor and normal tissue used by TCGA; ( f ) The landscape of DLGAP4-related clinicopathological features of HCC in the TCGA database. ( g - j ) DLGAP4 was significantly increased in higher AFP, higher-age, Female and HBV(+) HCC in theTCGA databases. The significance of the difference was tested by one-way ANOVA

Article Snippet: The sections were incubated overnight at 4 °C with a rabbit anti-human DLGAP4 antibody (1:500, Bioss, China).

Techniques: Expressing

Journal: Journal of Gastrointestinal Cancer

Article Title: Bioinformatics Analysis Reveals the Role of DLGAP4 in the Development and Progression of Hepatocellular Carcinoma

doi: 10.1007/s12029-025-01359-w

Figure Lengend Snippet: DLGAP4 is closely associated with transcription and translationimmune process regulation in HCC ( a - g ) Biological processes (BP) cellular components (CC) and molecular functions(MF) are mostly related to DLGAP4 in the TCGA database and GSE14520 . ( d - h ) Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of DLGAP4 in the TCGA database and GSE14520

Article Snippet: The sections were incubated overnight at 4 °C with a rabbit anti-human DLGAP4 antibody (1:500, Bioss, China).

Techniques:

Journal: Journal of Gastrointestinal Cancer

Article Title: Bioinformatics Analysis Reveals the Role of DLGAP4 in the Development and Progression of Hepatocellular Carcinoma

doi: 10.1007/s12029-025-01359-w

Figure Lengend Snippet: Correlation analysis between DLGAP4 expression and chromatin remodeling enrichment scores ( a - b ) The heatmap showed the expression of DLGAP4 and the enrichment scores of chromatin remodeling functions of each patient in the GSE14520 and The Cancer Genome Atlas (TCGA) databases. The samples were arranged in ascending order of the expression of DLGAP4. The column graph and line graph on the right showed the R-value and P-value of the correlation analysis

Article Snippet: The sections were incubated overnight at 4 °C with a rabbit anti-human DLGAP4 antibody (1:500, Bioss, China).

Techniques: Expressing

Journal: Journal of Gastrointestinal Cancer

Article Title: Bioinformatics Analysis Reveals the Role of DLGAP4 in the Development and Progression of Hepatocellular Carcinoma

doi: 10.1007/s12029-025-01359-w

Figure Lengend Snippet: The correlation between DLGAP4 expression and protein translation and chromatin remodeling in the GSE14520 and The Cancer Genome Atlas(TCGA)databases ( a ) Pearson correlation between DLGAP4 and protein translation. The width of the band represented the R-value. The color of the band represented the P-value. The correlation was tested by Pearson correlation analysis. ( b - c ) Correlation matrix of DLGAP4 and chromatin remodeling. The bottom left showed the correlation coefficient. The correlation coefficients were demonstrated as the proportion of the pie charts. The red parts represented a positive correlation, while the green parts represented a negative correlation.The correlation was tested by Pearson correlation analysis

Article Snippet: The sections were incubated overnight at 4 °C with a rabbit anti-human DLGAP4 antibody (1:500, Bioss, China).

Techniques: Expressing

Journal: Journal of Gastrointestinal Cancer

Article Title: Bioinformatics Analysis Reveals the Role of DLGAP4 in the Development and Progression of Hepatocellular Carcinoma

doi: 10.1007/s12029-025-01359-w

Figure Lengend Snippet: The expression pattern of DLGAP4 in HCC ( a and c ) Subtypes of lymphocytes from the surgical samples of HCC patients. Cell clustering from the surgical samples of HCC patients. ( b and d ) Association between DLGAP4 and Cancer cell markers(AFP, ALB, HNF4A and GPC3), stem cell markers (PROM1), tumor-associated macrophage markers (APOE and SLC40A1), Cancer-associated fibroblasts markers (ACTA2), angiogenesis markers (PECAM1), metabolic reprogramming (SQLE and ACSL4), cell cycle (CDKN3 and PRC1) of HCC

Article Snippet: The sections were incubated overnight at 4 °C with a rabbit anti-human DLGAP4 antibody (1:500, Bioss, China).

Techniques: Expressing

Journal: Journal of Gastrointestinal Cancer

Article Title: Bioinformatics Analysis Reveals the Role of DLGAP4 in the Development and Progression of Hepatocellular Carcinoma

doi: 10.1007/s12029-025-01359-w

Figure Lengend Snippet: Correlation analysis of DLGAP4 in tumor immune cells ( a - f ) Correlation analysis of DLGAP4 in CD4 + T cells, CD8 + T cells, B cells, dendritic cell, macrophage and neutrophil

Article Snippet: The sections were incubated overnight at 4 °C with a rabbit anti-human DLGAP4 antibody (1:500, Bioss, China).

Techniques:

Journal: Journal of Gastrointestinal Cancer

Article Title: Bioinformatics Analysis Reveals the Role of DLGAP4 in the Development and Progression of Hepatocellular Carcinoma

doi: 10.1007/s12029-025-01359-w

Figure Lengend Snippet: Kaplan-Meier analysis of DLGAP4 expression in the GSE14520 and The Cancer Genome Atlas (TCGA) databases ( a and b ) The cutoff of the group is the median expression of DLGAP4. The significance of the prognostic value was tested by a log-rank test

Article Snippet: The sections were incubated overnight at 4 °C with a rabbit anti-human DLGAP4 antibody (1:500, Bioss, China).

Techniques: Expressing

Journal: Journal of Gastrointestinal Cancer

Article Title: Bioinformatics Analysis Reveals the Role of DLGAP4 in the Development and Progression of Hepatocellular Carcinoma

doi: 10.1007/s12029-025-01359-w

Figure Lengend Snippet: Immunohistochemical detection of the expression of DLGAP4 and other proteins in liver cancer tissues ( a ) Immunohistochemical detection of the expression levels of DLGAP4, CD3, CD8, CD45RO, Foxp3 and Granzyme B. ( b - g ) Bar charts showing the results of various indicator tests.The significance of the difference was tested by one-way ANOVA

Article Snippet: The sections were incubated overnight at 4 °C with a rabbit anti-human DLGAP4 antibody (1:500, Bioss, China).

Techniques: Immunohistochemical staining, Expressing

Journal: Scientific Reports

Article Title: DLGAP4 acts as an effective prognostic predictor for hepatocellular carcinoma and is closely related to tumour progression

doi: 10.1038/s41598-022-23837-y

Figure Lengend Snippet: Interfering with DLGAP4 inhibits the PPARβ/δ signalling pathway and the expression of proliferation- and metastasis-related proteins. Western blotting was performed to measure the protein expression of DLGAP4, PPARβ/δ, CyclinD1, E-cadherin and N-cadherin in shNC or shDLGAP4 HepG2 and HCCLM3 cells. The data were obtained from the average of three independent experiments. *P < 0.05.

Article Snippet: The following antibodies were used: rabbit PPARβ/δ monoclonal antibody (1:2000, Abcam, Cambridge, MA, USA), mouse β-actin polyclonal antibody (1:1000, Boster Biological Technology, Ltd), rabbit E-cadherin polyclonal antibody (1:1000, Boster Biological Technology, Ltd), rabbit N-cadherin polyclonal antibody (1:1000, Boster Biological Technology, Ltd), rabbit DLGAP4 polyclonal antibody (1:1000, Abcam, Cambridge, MA, USA), and rabbit CyclinD1 polyclonal antibody (1:1000, Boster Biological Technology, Ltd.).

Techniques: Expressing, Western Blot